6ème Journées du Réseau "France Microtubules"

8:30 - 9:30 (1h)

arrival and registration

CBI entrance

9:30 - 9:40 (10min)

welcome address

amphitheatre CBI 0028

the organizers

9:40 - 10:40 (1h)

Microtubules and cell division

amphitheatre CBI 0028

› CELL DIVISION GEOMETRY IN TISSUE MORPHOGENESIS - Laura Ruiz, Institut de Biologie du Développement de Marseille 09:40-09:55 (15min)

› Insights into new forces involved in spindle positioning in Drosophila neural stem cells - Imane NAOUADIR, Institut de Génétique et Développement de Rennes 09:55-10:10 (15min)

› Role of microtubule-associated BORG2/CDC42EP3 in CDC42-dependent control of mitosis - Béatrice BENOIT, Université Paris-Saclay, UFR Pharmacie, Moissan, Orsay 10:10-10:25 (15min)

› Microtubule stiffening by doublecortin-domain protein ZYG-8 contributes to spindle orientation during C. elegans zygote division. - Hélène Bouvrais, Institut de Génétique et Développement de Rennes 10:25-10:40 (15min)

10:40 - 11:15 (35min)

Coffee break

CBI entrance

11:15 - 12:00 (45min)

Microtubule isotypes and posttranslational modifications

amphitheatre CBI 0028

› MAPping the β-Tubulin Tyrosination Landscape in Trypanosoma brucei via TurboID - JEREMY VINCENT, University of Montpellier, CNRS, IRD, MiVEGEC 11:15-11:30 (15min)

› Reduced tubulin polyglutamylation affects neuronal function and causes late-onset neurodegeneration - Shreyangi Chakraborty, Institut Curie, PSL Research University, CNRS UMR3348, Orsay, France, Université Paris-Saclay, CNRS UMR3348, Orsay, France 11:30-11:45 (15min)

› Tubulin isotypes polymerise into sectioned microtubules that locally regulate protein binding - Marcus Braun, Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, 25250 Vestec, Prague West 11:45-12:00 (15min)

12:00 - 13:00 (1h)

Lunch

13:00 - 14:15 (1h15)

poster session

CBI entrance

› Microtubule reorganization in epidermal keratinocytes - Keying GUO, Centre for Integrative Biology - CBI - Andreas MERDES, Centre for Integrative Biology - CBI 13:00-14:15 (1h15)

› Interplay between tubulin (de)tyrosination and autophagy in neurons and the brain. - Samar ISMAIL, Univ. Grenoble Alpes, Inserm, U1216, Grenoble Institut Neurosciences, GIN, 38000 Grenoble, France 13:00-14:15 (1h15)

› Spatial and temporal control of microtubule polyglutamylation in neurons - Eva Desvigne-Hansch, Intégrité du génome, ARN et cancer 13:00-14:15 (1h15)

› Elucidating β2-tubulin detyrosination and the tyrosination-dependent MAP code in Leishmania parasites - Rosa Milagros Corrales, University of Montpellier, CNRS, IRD, MiVEGEC 13:00-14:15 (1h15)

› The role of Microtubule-Associated Proteins in organising microtubule bundles in cells using expansion microscopy - 13:00-14:15 (1h15)

› Posttranslational encoding of microtubules provides unique recognition patterns for associated proteins - Arya Krishnan, Institut Curie, Université PSL, CNRS UMR3348, F-91401 Orsay, Université. Paris-Saclay, CNRS UMR3348, F-91401 Orsay 13:00-14:15 (1h15)

› The kinesin Kip2 promotes microtubule growth using a bipartite polymerase module to deliver tubulin to microtubule plus-ends - Didier Portran, Centre de recherche en Biologie cellulaire de Montpellier 13:00-14:15 (1h15)

› Quality control of spindle-associated proteins - Simos Nadalis, Centre de Recherche en Biologie cellulaire de Montpellier 13:00-14:15 (1h15)

› Determining the mechanistic roles of tubulin polyglutamylation in Purkinje-cell degeneration - 13:00-14:15 (1h15)

› Determining the mechanistic roles of tubulin glutamylation in the regulation of neuronal functions - Danilo Lopes, Institut Curie, Université Paris-Saclay 13:00-14:15 (1h15)

› VASH2-SVBP detyrosinating enzyme modulates microtubule dynamics - 13:00-14:15 (1h15)

› Role of tubulin (de)tyrosination in the Microtubule-Actin crosstalk in the dendritic spines - Aditi Sharma, Grenoble Institut des Neurosciences 13:00-14:15 (1h15)

› MBAPs link brassinosteroid signaling and cortical microtubules - Satoshi FUJITA, LRSV-Signalisation Cellulaire et Ubiquitination 13:00-14:15 (1h15)

› Remodelage des microtubules et du centrosome en quiescence - Aurélie Massoni-Laporte, Institut de biochimie et génétique cellulaires 13:00-14:15 (1h15)

14:15 - 15:30 (1h15)

Microtubules in differentiation and development

amphitheatre CBI 0028

› Asymmetric microtubule nucleation from Golgi stacks promotes opposite microtubule polarity in axons and dendrites - AKILA YAGOUBAT MERAH, Université Sorbonne Paris Cité 14:15-14:30 (15min)

› Cytoskeletal dynamics at synapses: characterization of novel microtubular regulators and their potential role in resilience to neurodegeneration - Martina Aleman, Univ. Grenoble Alpes, Inserm, U1216, Grenoble Institut Neurosciences, GIN, 38000 Grenoble, France 14:30-14:45 (15min)

› Regulation of focal adhesion turnover by the +TIP NAV1 during cell migration and axonal growth cone motility - Jérôme Boudeau, Centre de recherche en Biologie cellulaire de Montpellier 14:45-15:00 (15min)

› Centrosomal vs Golgi microtubules : Key players in osteoclast formation and function ? - Camille GORLT, Unité de biologie Moléculaire, Cellulaire et du Développement, Institut de pharmacologie et de biologie structurale 15:00-15:15 (15min)

› Centrosome and Microtubule Function in Dendritic Cells Orchestrate Adaptive Immune Activation - Eva Kiermaier, University of Bonn 15:15-15:30 (15min)

15:30 - 16:00 (30min)

Coffee break

CBI entrance

16:00 - 17:00 (1h)

Microtubules and diseases

amphitheatre CBI 0028

› A hypothetical functional amyloid of the tau protein - Benoit Gigant, Institut de Biologie Intégrative de la Cellule 16:00-16:15 (15min)

› Towards a new anti-cancer therapy targeting microtubules - Audrey Roussel, Centre de biophysique moléculaire 16:15-16:30 (15min)

› Tau condensation on microtubules generates specific microenvironment - Eva Lanska, Institute of Biotechnology of the Czech Academy of Sciences - Aniruddha Nagarajan, The Institute of Mathematical Sciences, Chennai, Tamil Nadu, India - Tereza Humhalova, Faculty of Science, Charles University, Prague, Czechia - Valerie Siahaan, Institute of Biotechnology of the Czech Academy of Sciences - Jochen Krattenmacher, Institute of Biotechnology of the Czech Academy of Sciences - Lenka Libusova, Faculty of Science, Charles University, Prague, Czechia - Carsten Janke, Institut Curie, PSL Research University,, Université Paris-Saclay - Zdenek Lansky, Institute of Biotechnology of the Czech Academy of Sciences - Marcus Braun, Institute of Biotechnology of the Czech Academy of Sciences - Sandeep Choubey, The Institute of Mathematical Sciences, Chennai, Tamil Nadu, India 16:30-16:45 (15min)

› PYRUVATE-MEDIATED MODULATION OF PRIMARY CILIA BIOLOGY IN COLONIC FIBROBLASTS - Maya Sarieddine, Institut de Génétique Moléculaire de Montpellier 16:45-17:00 (15min)

17:00 - 17:15 (15min)

Break

CBI entrance

17:15 - 18:15 (1h)

keynote lecture

amphitheatre CBI 0028

18:15 - 19:30 (1h15)

drinks and posters (max. width 90cm x height 135cm)

CBI entrance and IBCG seminar rooms

19:30 - 21:00 (1h30)

Dinner

	Thu. 26	Fri. 27
08:00 09:00 10:00 11:00 12:00 13:00 14:00 15:00 16:00 17:00 18:00 19:00 20:00 21:00	arrival and registration 8:30 - 9:30 (1h) arrival and registration CBI entrance welcome address 9:30 - 9:40 (10min) welcome address amphitheatre CBI 0028 the organizers Microtubules and cell division 9:40 - 10:40 (1h) Microtubules and cell division amphitheatre CBI 0028 › CELL DIVISION GEOMETRY IN TISSUE MORPHOGENESIS - Laura Ruiz, Institut de Biologie du Développement de Marseille 09:40-09:55 (15min) › Insights into new forces involved in spindle positioning in Drosophila neural stem cells - Imane NAOUADIR, Institut de Génétique et Développement de Rennes 09:55-10:10 (15min) › Role of microtubule-associated BORG2/CDC42EP3 in CDC42-dependent control of mitosis - Béatrice BENOIT, Université Paris-Saclay, UFR Pharmacie, Moissan, Orsay 10:10-10:25 (15min) › Microtubule stiffening by doublecortin-domain protein ZYG-8 contributes to spindle orientation during C. elegans zygote division. - Hélène Bouvrais, Institut de Génétique et Développement de Rennes 10:25-10:40 (15min) Coffee break 10:40 - 11:15 (35min) Coffee break CBI entrance Microtubule isotypes and posttranslational modifications 11:15 - 12:00 (45min) Microtubule isotypes and posttranslational modifications amphitheatre CBI 0028 › MAPping the β-Tubulin Tyrosination Landscape in Trypanosoma brucei via TurboID - JEREMY VINCENT, University of Montpellier, CNRS, IRD, MiVEGEC 11:15-11:30 (15min) › Reduced tubulin polyglutamylation affects neuronal function and causes late-onset neurodegeneration - Shreyangi Chakraborty, Institut Curie, PSL Research University, CNRS UMR3348, Orsay, France, Université Paris-Saclay, CNRS UMR3348, Orsay, France 11:30-11:45 (15min) › Tubulin isotypes polymerise into sectioned microtubules that locally regulate protein binding - Marcus Braun, Institute of Biotechnology, Czech Academy of Sciences, BIOCEV, 25250 Vestec, Prague West 11:45-12:00 (15min) Lunch 12:00 - 13:00 (1h) Lunch poster session 13:00 - 14:15 (1h15) poster session CBI entrance › Microtubule reorganization in epidermal keratinocytes - Keying GUO, Centre for Integrative Biology - CBI - Andreas MERDES, Centre for Integrative Biology - CBI 13:00-14:15 (1h15) › Interplay between tubulin (de)tyrosination and autophagy in neurons and the brain. - Samar ISMAIL, Univ. Grenoble Alpes, Inserm, U1216, Grenoble Institut Neurosciences, GIN, 38000 Grenoble, France 13:00-14:15 (1h15) › Spatial and temporal control of microtubule polyglutamylation in neurons - Eva Desvigne-Hansch, Intégrité du génome, ARN et cancer 13:00-14:15 (1h15) › Elucidating β2-tubulin detyrosination and the tyrosination-dependent MAP code in Leishmania parasites - Rosa Milagros Corrales, University of Montpellier, CNRS, IRD, MiVEGEC 13:00-14:15 (1h15) › The role of Microtubule-Associated Proteins in organising microtubule bundles in cells using expansion microscopy - 13:00-14:15 (1h15) › Posttranslational encoding of microtubules provides unique recognition patterns for associated proteins - Arya Krishnan, Institut Curie, Université PSL, CNRS UMR3348, F-91401 Orsay, Université. Paris-Saclay, CNRS UMR3348, F-91401 Orsay 13:00-14:15 (1h15) › The kinesin Kip2 promotes microtubule growth using a bipartite polymerase module to deliver tubulin to microtubule plus-ends - Didier Portran, Centre de recherche en Biologie cellulaire de Montpellier 13:00-14:15 (1h15) › Quality control of spindle-associated proteins - Simos Nadalis, Centre de Recherche en Biologie cellulaire de Montpellier 13:00-14:15 (1h15) › Determining the mechanistic roles of tubulin polyglutamylation in Purkinje-cell degeneration - 13:00-14:15 (1h15) › Determining the mechanistic roles of tubulin glutamylation in the regulation of neuronal functions - Danilo Lopes, Institut Curie, Université Paris-Saclay 13:00-14:15 (1h15) › VASH2-SVBP detyrosinating enzyme modulates microtubule dynamics - 13:00-14:15 (1h15) › Role of tubulin (de)tyrosination in the Microtubule-Actin crosstalk in the dendritic spines - Aditi Sharma, Grenoble Institut des Neurosciences 13:00-14:15 (1h15) › MBAPs link brassinosteroid signaling and cortical microtubules - Satoshi FUJITA, LRSV-Signalisation Cellulaire et Ubiquitination 13:00-14:15 (1h15) › Remodelage des microtubules et du centrosome en quiescence - Aurélie Massoni-Laporte, Institut de biochimie et génétique cellulaires 13:00-14:15 (1h15) Microtubules in differentiation and development 14:15 - 15:30 (1h15) Microtubules in differentiation and development amphitheatre CBI 0028 › Asymmetric microtubule nucleation from Golgi stacks promotes opposite microtubule polarity in axons and dendrites - AKILA YAGOUBAT MERAH, Université Sorbonne Paris Cité 14:15-14:30 (15min) › Cytoskeletal dynamics at synapses: characterization of novel microtubular regulators and their potential role in resilience to neurodegeneration - Martina Aleman, Univ. Grenoble Alpes, Inserm, U1216, Grenoble Institut Neurosciences, GIN, 38000 Grenoble, France 14:30-14:45 (15min) › Regulation of focal adhesion turnover by the +TIP NAV1 during cell migration and axonal growth cone motility - Jérôme Boudeau, Centre de recherche en Biologie cellulaire de Montpellier 14:45-15:00 (15min) › Centrosomal vs Golgi microtubules : Key players in osteoclast formation and function ? - Camille GORLT, Unité de biologie Moléculaire, Cellulaire et du Développement, Institut de pharmacologie et de biologie structurale 15:00-15:15 (15min) › Centrosome and Microtubule Function in Dendritic Cells Orchestrate Adaptive Immune Activation - Eva Kiermaier, University of Bonn 15:15-15:30 (15min) Coffee break 15:30 - 16:00 (30min) Coffee break CBI entrance Microtubules and diseases 16:00 - 17:00 (1h) Microtubules and diseases amphitheatre CBI 0028 › A hypothetical functional amyloid of the tau protein - Benoit Gigant, Institut de Biologie Intégrative de la Cellule 16:00-16:15 (15min) › Towards a new anti-cancer therapy targeting microtubules - Audrey Roussel, Centre de biophysique moléculaire 16:15-16:30 (15min) › Tau condensation on microtubules generates specific microenvironment - Eva Lanska, Institute of Biotechnology of the Czech Academy of Sciences - Aniruddha Nagarajan, The Institute of Mathematical Sciences, Chennai, Tamil Nadu, India - Tereza Humhalova, Faculty of Science, Charles University, Prague, Czechia - Valerie Siahaan, Institute of Biotechnology of the Czech Academy of Sciences - Jochen Krattenmacher, Institute of Biotechnology of the Czech Academy of Sciences - Lenka Libusova, Faculty of Science, Charles University, Prague, Czechia - Carsten Janke, Institut Curie, PSL Research University,, Université Paris-Saclay - Zdenek Lansky, Institute of Biotechnology of the Czech Academy of Sciences - Marcus Braun, Institute of Biotechnology of the Czech Academy of Sciences - Sandeep Choubey, The Institute of Mathematical Sciences, Chennai, Tamil Nadu, India 16:30-16:45 (15min) › PYRUVATE-MEDIATED MODULATION OF PRIMARY CILIA BIOLOGY IN COLONIC FIBROBLASTS - Maya Sarieddine, Institut de Génétique Moléculaire de Montpellier 16:45-17:00 (15min) Break 17:00 - 17:15 (15min) Break CBI entrance keynote lecture 17:15 - 18:15 (1h) keynote lecture amphitheatre CBI 0028 drinks and posters (max. width 90cm x height 135cm) 18:15 - 19:30 (1h15) drinks and posters (max. width 90cm x height 135cm) CBI entrance and IBCG seminar rooms Dinner 19:30 - 21:00 (1h30) Dinner	Microtubule assembly 9:15 - 10:30 (1h15) Microtubule assembly amphitheatre CBI 0028 › Beyond mitosis: targeted degradation uncovers novel interphase functions of augmin. - Marcos Fariña-Mosquera, Institute for Research in Biomedicine [Barcelona, Spain] 09:15-09:30 (15min) › C- and D-type lateral interactions weaken the microtubule lattice - Matthieu Benoit, Institut de Génétique et Développement de Rennes (IGDR) UMR 6290, MiToS team 09:30-09:45 (15min) › Mechanistic Dissection of γTuRC Activation by Centrosomin Motif 1 (CM1) - Cláudia Fernandes Brito, Centro de Regulación Genómica 09:45-10:00 (15min) › Xenopus spermatid microtubules contain an internal helix - Céline Callens, Institut de Génétique et Développement de Rennes 10:00-10:15 (15min) › Stable GDP-tubulin islands rescue dynamic microtubules - Virginie Stoppin-Mellet, Grenoble Institut des Neurosciences 10:15-10:30 (15min) Coffee break 10:30 - 11:00 (30min) Coffee break CBI entrance Microtubule-dependent motors and transport 11:00 - 12:00 (1h) Microtubule-dependent motors and transport amphitheatre CBI 0028 › Precise control of microtubule structure and intracellular trafficking in living cells and behaving animals - Yu-Chun Lin, National Tsing Hua University [Hsinchu] 11:00-11:15 (15min) › Spatial activation of Kinesin-1 by Ensconsin shapes microtubule networks via ncMTOCs recruitment - Laetitia Bataillé, Institut de Génétique et Développement de Rennes (IGDR), Univ Rennes, CNRS, UMR 6290, INSERM ERL U1305 11:15-11:30 (15min) › NuMA is a mitotic adaptor protein that activates dynein and connects it to microtubule minus ends - Sabina Colombo, Centro de Regulación Genómica (CRG) 11:30-11:45 (15min) › Kinesin-12 KLP-18 contributes to the kinetochore-microtubule poleward flux during the metaphase of C. elegans one-cell embryo. - Mathis Da Silva, Institut de Génétique et Développement de Rennes 11:45-12:00 (15min) Lunch 12:00 - 14:00 (2h) Lunch Microtubule inhibitors 14:00 - 14:45 (45min) Microtubule inhibitors amphitheatre CBI 0028 › A new nanoDSF approach to anti-tubulin compounds screening - Philipp TSVETKOV, Institut de neurophysiopathologie 14:00-14:15 (15min) › Effects of oxfendazole, a microtubule-targeting agent, on the development of filarial parasites - Linda KOHL, UMR7245 MCAM, MNHN-CNRS 14:15-14:30 (15min) › Peptide-based covalent inhibitor of tubulin detyrosination promotes mesenchymal-to-epithelial transition in lung cancer cells - Ghislain Gillard, Institut de Génétique Humaine 14:30-14:45 (15min) prizes for junior presenters, planning of future meetings, closing remarks 14:45 - 15:30 (45min) prizes for junior presenters, planning of future meetings, closing remarks amphitheatre CBI 0028